In vitro effects of oxygenated lanosterol derivatives on cholesterol biosynthesis from 24,25-dihydrolanosterol.
- 1 January 1988
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 36 (3) , 966-973
- https://doi.org/10.1248/cpb.36.966
Abstract
The effects of oxygenated lanosterol derivatives (1-27, 5 .mu.M) including 32-oxygenated lanosterol derivatives on cholesterol biosynthesis from [24,25-3H2]-24,25-dihydrolanosterol (18 .mu.M) were tested in 10,000 .times. g supernatant (S-10) fraction of rat liver homogenate. Among the derivatives, 7-oxolanost-8-en-3.beta.-ol (7-oxo-DHL), 3.beta.-acetoxylanost-8-en-7-one (7-oxo-DHL-3-OAc), and 7-oxolanosta-5,8,11-trien-3.beta.-ol were highly active in depressing cholesterol biosynthesis from 24,25-dihydrolanosterol. The inhibitory activities of these derivatives on cholesterol synthesis are discussed on the basis of the position and stereochemistry of the oxygen functional groups on the sterol nucleus. The effect of aphidicolin on cholesterol synthesis was also compared with that of 7-oxo-DHL.This publication has 3 references indexed in Scilit:
- Metabolism of 32-hydroxy-24,25-dihydrolanosterol by purified cytochrome P-45014DM from yeast. Evidence for contribution of the cytochrome to whole process of lanosterol 14 alpha-demethylation.Journal of Biological Chemistry, 1987
- Inhibition, post-hydroxymethylglutaryl-CoA regulation and relation to cell growth of cholesterol biosynthesis in cultured human skin fibroblastsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1985
- Synthesis of lanosterol analogs with lengthened side chains and their effects on cholesterol biosynthesis from lanosterol.CHEMICAL & PHARMACEUTICAL BULLETIN, 1984