A simple immunochemical technique for distinguishing melanocytes and melanophages in paraffin-embedded tissue

Abstract
We describe an immunoperoxidase technique for distinguishing melanocytes and melanophages in paraffin-embedded tissue. Two primary antibodies were used: anti-S100 and HAM-56, a monoclonal antibody directed against macrophages. In a variety of pigmented lesions, HAM-56 labelled melanophages while S100 labelled cells of melanocytic lineage.