The Stereochemical Course of Group II Intron Self-Splicing
- 9 December 1994
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 266 (5191) , 1685-1688
- https://doi.org/10.1126/science.7527587
Abstract
The stereochemical specificities and reaction courses for both self-splicing steps of a group II intron have been determined by phosphorothioate substitution at the 5' and 3' splice site phosphodiester bonds. Both steps of the splicing reaction proceeded with a phosphorothioate in the Sp configuration but were blocked by the Rp diastereomer. Both steps also proceeded with inversion of stereochemical configuration around phosphorus, consistent with a concerted transesterification reaction. These results are identical to those found for nuclear precursor mRNA (pre-mRNA) splicing and provide support for the hypothesis that group II introns and nuclear pre-mRNA introns share a common evolutionary history.Keywords
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