Stereochemical Course of Catalysis by the Tetrahymena Ribozyme
- 12 May 1989
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 244 (4905) , 692-694
- https://doi.org/10.1126/science.2470151
Abstract
The group I intron from Tetrahymena catalyzes phosphodiester transfer reactions on various RNA substrates. A modified RNA substrate with a phosphorothioate group in one stereoisomeric form at the site of reaction was synthesized in order to determine the stereochemical course of an RNA-catalyzed reaction. The reaction product was digested with a stereospecific nuclease to determine the configuration of the product phosphorothioate. The reaction occurs with inversion of configuration at phosphorus, implying an in-line pathway for the reaction.This publication has 16 references indexed in Scilit:
- The Chemistry of Self-Splicing RNA and RNA EnzymesScience, 1987
- The Tetrahymena ribozyme acts like an RNA restriction endonucleaseNature, 1986
- The Tetrahymena intervening sequence ribonucleic acid enzyme is a phosphotransferase and an acid phosphataseBiochemistry, 1986
- Enzymatic activity of the conserved core of a group I self-splicing intronNature, 1986
- The Intervening Sequence RNA of Tetrahymena Is an EnzymeScience, 1986
- NUCLEOSIDE PHOSPHOROTHIOATESAnnual Review of Biochemistry, 1985
- Specific interaction between the self-splicing RNA of Tetrahymena and its guanosine substrate: implications for biological catalysis by RNANature, 1984
- Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymenaCell, 1982
- Unambiguous determination of the stereochemistry of nucleotidyl transfer catalyzed by DNA polymerase I from Escherichia coliBiochemistry, 1981
- Enzyme-Catalyzed Phosphoryl Transfer ReactionsAnnual Review of Biochemistry, 1980