The expression of human plasma cholesteryl‐ester‐transfer protein in HepG2 cells in induced by sodium butyrate
Open Access
- 1 December 1993
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 218 (3) , 945-950
- https://doi.org/10.1111/j.1432-1033.1993.tb18451.x
Abstract
Although human plasma cholesteryl‐ester‐transfer protein (CETP) is primarily synthesized in the liver, its expression in a number of transformed liver cell lines is very low. However the use of the human hepatoma cell line HepG2 as a model system for the regulation of CETP on mRNA level is facilitated by a quantitative reverse‐transcribed polymerase chain reaction. We demonstrate a time‐dependent and concentration‐dependent 3–4‐fold induction of CETP mRNA by sodium butyrate. CETP mass in the medium is also augmented; however, the effect on protein level is less pronounced.Keywords
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