Effects of recombinant human IL-I? on production of prostaglandin E2, leukotriene B4, NAG, and superoxide by human synovial cells and chondrocytes

Publisher Website
Google Scholar
Abstract
The effects of recombinant human IL-1β on the production of prostaglandin E2 (PGE2), leukotriene B4 (LTB4),N-acetyl-β-D-glucosaminidase (NAG), and superoxide by synovial cells and chondrocytes derived from osteoarthritis patients were determined. IL-1β markedly enhanced PGE2 production in chondrocytes and, to the lesser extent, in synovial cells. Synovial cells and chondrocytes spontaneously released LTB4 into culture medium and IL-1β significantly inhibited LTB4 production by these cells. IL-1β significantly suppressed the release of NAG and superoxide by synovial cells, whereas it significantly enhanced the production of NAG and superoxide by chondrocytes. Production of intracellular superoxide dismutase by synovial cells was significantly enhanced on incubation with IL-1β, but that of chondrocytes was not altered. IL-6, unlike IL-1β, significantly suppressed the production of NAG and superoxide by synovial cells and chondrocytes. These results suggest that IL-1 has differing effects on the release of mediators by synovial cells and chondrocytes and that these cells also vary in their responses to IL-1β and IL-6.