Scanning from an independently specified branch point defines the 3′ splice site of mammalian introns
- 1 November 1989
- journal article
- Published by Springer Nature in Nature
- Vol. 342 (6247) , 243-247
- https://doi.org/10.1038/342243a0
Abstract
During pre-messenger RNA splicing the lariat branch point in mammalian introns is specified independently of the 3' splice site by the sequence surrounding the branch point and by an adjacent downstream polypyrimidine tract. The 3' splice site is dispensable for spliceosome assembly and cleavage at the 5' splice site, and is itself determined by a scanning process that recognizes the first AG located 3' of the branch point/polypyrimidine tract, irrespective of distance or sequence environment.Keywords
This publication has 45 references indexed in Scilit:
- Signals for the selection of a splice site in pre-mRNAJournal of Molecular Biology, 1987
- The role of small nuclear ribonucleoprotein particles in pre-mRNA splicingNature, 1987
- PRE-mRNA SPLICINGAnnual Review of Genetics, 1986
- A compensatory base change in U1 snRNA suppresses a 5′ splice site mutationCell, 1986
- SPLICING OF MESSENGER RNA PRECURSORSAnnual Review of Biochemistry, 1986
- U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicingCell, 1985
- A multicomponent complex is involved in the splicing of messenger RNA precursorsCell, 1985
- The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent ReactionScience, 1985
- A point mutation in the conserved hexanucleotide at a yeast 5′ splice junction uncouples recognition, cleavage, and ligationCell, 1985
- A catalogue of splice junction sequencesNucleic Acids Research, 1982