Accurate determination of microbial diversity from 454 pyrosequencing data
Top Cited Papers
- 9 August 2009
- journal article
- Published by Springer Nature in Nature Methods
- Vol. 6 (9) , 639-641
- https://doi.org/10.1038/nmeth.1361
Abstract
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.Keywords
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