A new nitrogen-fixing species of pseudomonad: Pseudomonas diazotrophicus sp. nov. isolated from the root of wetland rice

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Abstract
A new rod-shaped, Gram-negative, polarly flagellated, and facultatively chemolithotrophic nitrogen-fixing bacterium isolated from the roots of rice (Oryza sativa) was described. The bacterium fixed nitrogen (N2), used at least 55 organic compounds as sole carbon source, and did not require growth factors. It consisted of at least two distinct serogroups. The deoxyribonucleic acid base composition was 65.1–67.3 mol% G+C. The quinone system consisted of coenzyme Q10 as a major ubiquinone and coenzyme Q9 as a minor component. Cellular fatty acids consisted of 63–85% cw-9-octadecenoic acid, 7–10% octadecanoic acid, and 1–7% 3-hydroxytetradecanoic acid. These isolates are considered typical of a new species in the genus Pseudomonas and the proposed name is Pseudomonas diazotrophicus sp. nov.
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