Urea Decomposition as a Means of Differentiating Proteus and Paracolon Cultures from Each Other and from Salmonella and Shigella Types

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Abstract
A medium was devised for detecting urease activity of organisms unable to utilize ammonia as a sole source of N. This medium consisted of 0.1% peptone, 0.5% NaCI, 0.2% monopotassium phosphate, 0.0012% phenol red, 0.1% glucose, 2% urea and 2% agar. An intense red-violet color indicates urea decomposition. This medium showed that, in addition to Proteus, paracolon Aerobacter and paracolon intermediates were urease-positive. Paracolon Escher-ichia, Salmonella and Shigella spp. were urease-negative.

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