Rate of Elongation of Polyphenylalanine in vitro

Publisher Website
Google Scholar
Abstract
Ribosomes purified from Escherrchipa coli were preincubated with AcPhe‐tRNA and poly(U). Then purified components necessary for polypeptide synthesis were added. Incubation of the complete system led to a burst of elongation which lasted for nearly 10 s. During the initial burst approximately 10% of the ribosomes participated in the elongation of poly(Phe) at an average rate per ribosome close to eight peptide bonds/s. The missense error rate with leucine was 4x10−4 during the burst. Accordingly, the preincubated elongation system functions at a rate, as well as an accuracy, close to those of protein synthesis in vivo.