Role of Pancreatic Cholesterol Esterase in the Uptake and Esterification of Cholesterol by Isolated Intestinal Cells

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Abstract
Isolated intestinal mucosal cells from normal and common duct-cannulated rats were prepared by collagenase dissociation. These cells displayed ultrastructural integrity and retained several metabolic characteristics typical of intact intestinal mucosa. These included galactose accumulation and its inhibition by dinitrophenol, glucose oxidation, incorporation of labeled leucine into cellular protein and the the uptake and esterification of fatty acid. The uptake of cholesterol by cells from common duct-cannulated rats was comparable to that in cells from controls; however, esterification was only about 25% of that in control cells. Preincubation of the defective cells for 30 min with the purified subunit or active form of pancreatic sterol ester hydrolase had no effect on cholesterol uptake but resulted in a 4- to 6-fold increase in the ability of cells to esterify cholesterol. Additional evidence for the essential role of pancreatic sterol ester hydrolase in the mucosal esterification of absorbed cholesterol prior to lymphatic transport is presented.

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