Changes in the receptor for immunoglobulin E coincident with receptor-mediated stimulation of basophilic leukemia cells

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Abstract
Aggregation of the receptor for IgE on mast cells and related tumor cells initiates exocytosis. Tumor cells that had incorporated [3H] Leu and 32P were examined to see if stimulating them produced modifications in the receptors themselves. No changes were observed in the yield of receptors or in the relative proprotion and the molecular weights of their .alpha., .beta. and .gamma. subunits. No new receptor-associated components were observed. After the cells were stimulated, the .gamma. chains of the receptors showed an average 35% decrease in their associated 32P. Changes in the .beta. subunits were more variable but on the average showed a similar-sized increase in 32P. Using a novel protocol that permitted examination of aggregated and unaggregated receptors from the same cell, changes in the unaggregated receptors were quantitatively indistinguishable from those exhibited by the aggregated receptors. The changes maybe related to 1 of the inactivation reactions thought to accompany the activation sequence.