Dehydrogenase enzyme cytochemistry of unfixed leucocytes

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Abstract
An inert, synthetic, sucrose polymer (Ficoll) has been found to have a protective effect on leucocytes during incubation for cytochemical reactions at 37°C. This permits enzyme cytochemistry to be performed on unfixed bone marrow and peripheral blood smears, and on leucocytes cultured in vitro, thus avoiding enzyme inhibition by the use of a fixative. The technique is suitable for the demonstration of both cytoplasmic and intramitochondrial dehydrogenase enzyme activity.