CHROMATOGRAPHIC SEPARATION OF THE SODIUM-RETAINING CORTICOID FROM THE URINE OF CHILDREN WITH NEPHROSIS, COMPARED WITH OBSERVATIONS ON NORMAL CHILDREN 12

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Abstract
Lipid extracts of urine of 5 children with nephrosis were fractionated by paper chromatography. The Na-retaining activity of these extracts resides in a fraction which is presumably of adrenal cortical origin. This material has a specific Na-retaining activity 15-20 times as great as that of desoxycorticosterone. None of the known adrenal cortical steroids or their derivatives thus far tested shows the high specific activity and similar chromatographic behavior of the Na-retaining material in urine. The highly active mineral-ocorticoid of adrenal cortical extract described by Tait and Simpson appears to be very similar, if not identical, to the Na retaining corticoid of urine. Available information suggest that the active material is an 11-desoxycorticosteroid with 1 or more oxygen-containing substituents. Extracts of urine from 4 normal children on an unrestricted dietary intake of Na were chromatographed. Neither the extracts nor any chromatographic fraction showed significant Na-retaining activity.