An Evaluation of Eluent Recycling and Column Life for HPLC Analysis of Carbohydrates
- 30 April 1983
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 6 (6) , 1139-1151
- https://doi.org/10.1080/01483918308076096
Abstract
The length of service of amine modified silica columns for the automated analysis of carbohydrates in food and beverages typically exceeds 500 sample injections. Excessive accumulation of carbohydrate in the recirculated eluent results in a gradual decrease in peak height. Detector response is independent of injection volume over the range of 1–50 μl. These results suggest critical parameters for use in automated carbohydrate analysis systems.This publication has 8 references indexed in Scilit:
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