Alkylation of Reduced κ-Casein

Abstract
The alkylation of reduced [KAPPA] -casein with acrylonitrile and iodoacetamide results in the complete conversion of disulfides and/or thiol groups to S-alkylcystinyl residues under specified conditions. Acrylonitrile alkylates up to 45% of the lysine residues, whereas iodoacetamide alkylates only 7% of the lysine residues. No other amino-acids are affected by either alkylating agent.

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