c-myc mRNA in cytoskeletal-bound polysomes in fibroblasts
- 1 March 1991
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 274 (2) , 607-609
- https://doi.org/10.1042/bj2740607
Abstract
3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, 130 mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in order to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta 2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.Keywords
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