A simple and generally applicable procedure for releasing DNA from bacterial cells
- 1 May 1986
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 51 (5) , 1138-1140
- https://doi.org/10.1128/aem.51.5.1138-1140.1986
Abstract
Treatment of Staphylococcus simulans biovar staphylolyticus cells with acetone before digestion with lysozyme made the cells susceptible to lysis by sodium dodecyl sulfate. This technique was found to be useful for releasing DNA from a wide variety of gram-positive and gram-negative organisms. ImagesThis publication has 14 references indexed in Scilit:
- [17] A rapid alkaline extraction method for the isolation of plasmid DNAPublished by Elsevier ,1983
- Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosomeJournal of Bacteriology, 1982
- A general method for isolation of high molecular weight DNA from eukaryotesNucleic Acids Research, 1976
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970
- The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. The effects of changes in conformationBiochemical Journal, 1969
- Use of Lysostaphin in the Isolation of Highly Polymerized Deoxyribonucleic Acid and in the Taxonomy of Aerobic MicrococcaceaeJournal of Bacteriology, 1968