Morphologic, immunologic, enzymehistochemical and chromosomal analysis of a cell line derived from Hodgkin's disease. Evidence for a B-cell origin of sternberg-reed cells

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Abstract
Cell lines derived from Hodgkin's disease may provide a clue to the nature of Sternberg‐Reed cells. In the current study, the establishment of an Epstein‐Barr‐virus‐negative lymphoblastoid cell line, derived from the pleural fluid of a patient with the nodular sclerosis type of Hodgkin's diseae, is described. The morphologic and immunologic cell marker findings indicate that this cell line is derived from Sternberg‐Reed cells. The immunologic findings and a chromosomal analysis are in agreement with a B‐lymphocyte origin of these cultured cells. Extrapolation of the results to Hodgkin's disease in vivo would indicate that Hodgkin's disease, like most non‐Hodgkin's lymphomas, is the result of B‐cell proliferation.