OXIDATIVE ASSIMILATION BY AZOTOBACTER AGILIS

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Abstract
O2 consumption, CO2 production, and C assimilation values were determined in suspensions and in cultures of A. agilis. Assimilation values noted in suspensions of washed cells were approximately 14% for glucose, 18% for acetate or succinate, and 22% for lactate or pyruvate. Dinitrophenol or sodium azide had no significant effect on the extent of assimilation. The extent of glucose assimilation by growing cells was of the order of 10 to 15% as estimated by respiratory exchange, increase in cellular carbon, or increase in radioactivity when uniformly labeled glucose was employed. Tracer studies indicate that more of the C2-carbon than the C1-carbon of glucose was assimilated, suggesting that a major portion of the glucose is utilized over a pathway other than the Embden-Meyerhof one.
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