Characterization of the 97 and 103 kDa forms of starch branching enzyme from potato tubers
- 11 October 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 332 (1-2) , 132-138
- https://doi.org/10.1016/0014-5793(93)80499-k
Abstract
N-Terminal analysis, peptide mapping and partial peptide sequencing of the 97 and 103 kDa forms of starch branching enzyme from potato tubers showed that the two forms are highly related. A comparison with sequence data in the literature showed that these forms belong to the starch branching enzyme isoform I family. An internal cDNA fragment was obtained using PCR technology on potato tuber RNA with two oligonucleotide primers constructed from the peptide sequence data. Southern blot analysis using the PCR fragment as probe showed that there is only one gene locus encoding this isoform of the enzyme in Solanum tuberosum as well as in Solanum commersonii.Keywords
This publication has 20 references indexed in Scilit:
- Characterisation of the (1→4)-α-d-glucan-branching 6-glycosyltransferase by in vitro synthesis of branched starch polysaccharidesCarbohydrate Research, 1992
- Purification of two forms of starch branching enzyme (Q‐enzyme) from developing rice endospermPhysiologia Plantarum, 1992
- Sequence conservation of the catalytic regions of anylolytic enzymes in maize branching enzyme-IBiochemical and Biophysical Research Communications, 1991
- Isolation of a Q-Enzyme with Mr 103 000 from potato tubersPhytochemistry, 1991
- Immunological Comparison of the Starch Branching Enzymes from Potato Tubers and Maize KernelsPlant Physiology, 1989
- Maize leaf and kernel starch synthases and starch branching enzymesPhytochemistry, 1988
- Soluble starch synthases and starch branching enzymes from developing seeds of sorghumPhytochemistry, 1985
- Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and β2-microglobulinCell, 1979
- Purification and Properties of Potato 1,4‐α‐d‐Glucan: 1,4‐α‐d‐Glucan 6‐α‐(1,4‐α‐Glucano)‐TransferaseEuropean Journal of Biochemistry, 1975
- Corn and potato α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase. Evidence for separate hydrolytic and branching componentsBiochemistry, 1971