Specific Primers for Rapid Detection of Microsporum audouinii by PCR in Clinical Samples

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Abstract
This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA) 4 , and (GTG) 5 . The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii , which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of M. audouinii . These primers produced a singular PCR band of 431 bp specific only to strains and isolates of M. audouinii , based on a global test of 182 strains/isolates belonging to 11 species of dermatophytes. These findings indicate these primers are reliable for diagnostic purposes, and we recommend their use in laboratory analysis.