A LISS spin enzyme method for the detection of red cell antibodies and its use in routine antibody screen procedures

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Abstract
A technique for performing the enzyme phase of the antibody screen on red cells suspended in low‐ionic‐strength‐salt solution (LISS) is described. The reliability of this LISS spin‐enzyme (LSE) technique was compared with the two‐stage papain‐tile method, in the detection of 62 previously identified enzyme reacting antibodies. All 62 antibodies examined were detected by the LSE method, and no false‐positive reactions were found. Using LSE and papain‐tile methods in parallel, further assessment was obtained by screening 2000 sequential blood samples under routine service conditions. Fifty‐six blood samples contained alloantibodies, of which 43 reacted by both methods, eight by the LSE method only, and five by the papain‐tile method only. It was concluded that the LSE method was comparable to the papain‐tile method.