Interlocked Feedback Loops Within the Drosophila Circadian Oscillator
- 22 October 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 286 (5440) , 766-768
- https://doi.org/10.1126/science.286.5440.766
Abstract
Drosophila Clock (dClk) is rhythmically expressed, with peaks in mRNA and protein (dCLK) abundance early in the morning. dClk mRNA cycling is shown here to be regulated by PERIOD-TIMELESS (PER-TIM)–mediated release of dCLK- and CYCLE (CYC)–dependent repression. Lack of both PER-TIM derepression and dCLK-CYC repression results in high levels of dClk mRNA, which implies that a separate dClk activator is present. These results demonstrate that the Drosophila circadian feedback loop is composed of two interlocked negative feedback loops: aper-tim loop, which is activated by dCLK-CYC and repressed by PER-TIM, and a dClk loop, which is repressed by dCLK-CYC and derepressed by PER-TIM.Keywords
This publication has 22 references indexed in Scilit:
- The Drosophila Clock Gene double-time Encodes a Protein Closely Related to Human Casein Kinase IεCell, 1998
- Role of the CLOCK Protein in the Mammalian Circadian MechanismScience, 1998
- Robust circadian rhythmicity of Drosophila melanogaster requires the presence of lateral neurons: a brain-behavioral study of disconnected mutantsJournal of Comparative Physiology A, 1998
- Post-transcriptional regulation contributes to Drosophila clock gene mRNA cyclingThe EMBO Journal, 1997
- Independent Photoreceptive Circadian Clocks Throughout DrosophilaScience, 1997
- Temporally regulated nuclear entry of the Drosophila period protein contributes to the circadian clockNeuron, 1995
- Loss of Circadian Behavioral Rhythms and per RNA Oscillations in the Drosophila Mutant timelessScience, 1994
- Temporal phosphorylation of the Drosophila period protein.Proceedings of the National Academy of Sciences, 1994
- A promoterless period gene mediates behavioral rhythmicity and cyclical per expression in a restricted subset of the drosophila nervous systemNeuron, 1994
- Feedback of the Drosophila period gene product on circadian cycling of its messenger RNA levelsNature, 1990