Application of freeze-dried, one-day-old chick erythrocytes to viral hemagglutination and hemagglutination-inhibition tests

Publisher Website
Google Scholar
Abstract
Erythrocytes collected from 1 day old chicks were stabilized by fixation with formaldehyde and by freeze-drying after treatment with CO. Suspensions of freeze-dried erythrocytes in distilled water or physiological saline had a homogeneous bright reddish-purple color. Freeze-dried erythrocytes were compared with fresh erythrocytes for hemagglutination and hemagglutination inhibition tests for various viruses including rubella, Japanese encephalitis, influenza, mumps, Newcastle disease and Sendai viruses. After storage at 4.degree. C for .gtoreq. 1 yr, freeze-dried erythrocytes maintained their original appearance and sensitivity to hemagglutination antigens.