Chromatographic methods for the study of amines from biological material

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Abstract
A method has been developed for treating biological fluids and extracts with ion-exchange resins to prepare a fraction consisting predominantly of the amines. The resolution of this fraction has been obtained by the use of buffered columns of a weakly acidic ion-exchange resin. Amines separated in this way have been isolated as their hydrochlorides by a chromatographic desalting technique, and identified by paper chromatography and paper electrophoresis. The scope of these methods is illustrated with reference to the analysis of amines in urine, and figures are presented for the excretion of piperidine, dimethylamine and methylamine, both normally and after fasting.