Analysis of a Gene Cluster ofEnterococcus faecalisInvolved in Polysaccharide Biosynthesis
- 1 February 2000
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 68 (2) , 815-823
- https://doi.org/10.1128/iai.68.2.815-823.2000
Abstract
Previously, we described a gene cluster ofEnterococcus faecalisOG1RF that produced an antigenic polysaccharide when cloned inEscherichia coli. The polysaccharide antigen was not detectable inE. faecalisstrains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption oforfde4, encoding a putative glycosyl transferase, andorfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoidE. faecalisisolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.Keywords
This publication has 27 references indexed in Scilit:
- Pseudomonas aeruginosa in cystic fibrosis: role of mucC in the regulation of alginate production and stress sensitivityMicrobiology, 1997
- Molecular biology of the capsular genes of Streptococcus pneumoniaeFEMS Microbiology Letters, 1997
- Regulation of capsule synthesis includes interactions of the RcsC/RcsB regulatory pairResearch in Microbiology, 1994
- Differentiation of Pseudomonas aeruginosa into the alginate‐producing form: inactivation of mucB causes conversion to mucoidyMolecular Microbiology, 1993
- Biosynthesis and Expression of Cell-Surface Polysaccharides in Gram-Negative BacteriaPublished by Elsevier ,1993
- The Genetics of Encapsulation in Haemophilus influenzaeThe Journal of Infectious Diseases, 1992
- Biotyping, serotyping and phage typing of Streptococcus faecalis isolated from dental plaque in the human mouthJournal of Medical Microbiology, 1987
- Generalized transduction by phage P22 in Salmonella typhimurium: I. Molecular origin of transducing DNAJournal of Molecular Biology, 1972
- THE CELL WALLS OF GROUP D STREPTOCOCCIThe Journal of Experimental Medicine, 1965
- The Serological Typing of Group D Streptococci associated with Outbreaks of Neonatal DiarrhoeaJournal of General Microbiology, 1952