IMMUNE HAEMOLYSIS IN AGAR - DEMONSTRATION OF PROTECTIVE ACTION OF ANTIBODIES

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  • 1 January 1967
    • journal article
    • research article
    • Vol. 13  (4) , 411-+
Abstract
Certain human sera contain antibodies which under some circumstances inhibit complement dependent lysis of sensitized sheep cells. The activity of these antibodies was demonstrated by the use of agarose plates containing sensitized sheep cells. Most human sera placed in small holes cut in the plates produced homogeneous zones of total lysis around the holes. Occasional sera produced an inner zone of unlysed cells within an outer zone of lysis. Detailed studies of 3 such sera showed that this appearance was due to a protective effect of natural anti-sheep cell antibodies of IgG [immunoglobulin G] class.