Comparison of Buoyant Density of Two Mutants of ECHO 6 by Density Gradient Ultracentrifugation.

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Abstract
Mutants of ECHO-6 virus ([image] + and [image]) from Charles strain, were ultracentrifuged separately and as mixtures in a CsCl density gradient. Centrifugation was generally performed at 36,000 rpm for 41-46 hrs. and in 1 experiment at 25,000 rpm for 72 hrs. Five-drop and 1-drop fractions were collected and assayed for in-fectivity by plaque formation in primary rhesus monkey kidney (PRMK) or in a continuous line of African green monkey kidney (BGM) cell cultures. Densities corresponding to the maxima of the major infectivity peak for each mutant were compared. A small different in buoyant density in the range of 0.001 - 0.004 g/cm3 was determined, with the [image] mutant being heavier than the [image] + mutant. This small difference might reflect a real difference in composition of the particles or a difference in binding of Cs+ ion, or components of the cell culture system.

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