IMMUNOLOGIC STUDIES OF GLYCINE-INDUCED SPHEROPLASTS OF SALMONELLA TYPHI

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Abstract
The serological activity of glycine-induced spheroplasts and normal intact cells of Salmonella typhi was assessed. No major differences could be detected in the somatic O and in the Vi antigens of spheroplasts and intact cells. The only major change associated with transformation of normal typhoid organisms into spheroplasts seems to be associated with the flagellar antigen. Spheroplasts evoked a high H agglutinin response in immunized rabbits but were poorly agglutinated by standard H antiserum.The immunogenic ability of the spheroplasts to protect mice from the typhoid challenge was about five times higher than that of intact cells and the toxicity was reduced at least 10-fold.