High‐throughput single nucleotide polymorphism typing by fluorescent single‐strand conformation polymorphism analysis with capillary electrophoresis
- 1 March 2004
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 25 (6) , 833-838
- https://doi.org/10.1002/elps.200305721
Abstract
In this study, we performed high‐throughput and precise single nucleotide polymorphism (SNP) typing by fluorescent capillary electrophoresis single‐strand conformation polymorphism (CE‐SSCP) analysis. A system composed of a multicapillary DNA analyzer, a newly developed sieving matrix, four different colors of fluorescent labels, and a multiplex polymerase chain reaction (PCR) enabled low‐cost and highly reliable SNP typing. Moreover, this system enabled the estimation of SNP allele frequencies using pooled DNA samples, which should be beneficial for large‐scale association studies. Thus, fluorescent CE‐SSCP analysis is a useful method for large‐scale SNP typing.Keywords
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