"De Novo" Sequencing of Peptides Recovered from In-Gel Digested Proteins by Nanoelectrospray Tandem Mass Spectrometry
- 1 January 2002
- journal article
- Published by Springer Nature in Molecular Biotechnology
- Vol. 20 (1) , 107-118
- https://doi.org/10.1385/mb:20:1:107
Abstract
Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.Keywords
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