PNA∗Synthesis and Diagnostic Application
- 1 July 1997
- journal article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 16 (7) , 1771-1774
- https://doi.org/10.1080/07328319708006275
Abstract
An optimized automated PNA synthesis protocol is reported. Under optimal conditions the product yield of a test 17-mer PNA is approximately 90 %. The average coupling yield is 99.4 %. The synthesis strategy is Boc/Z. The protocol is developed in a 5 pmole scale but is easily scaled up to 10–50 μmole scale syntheses on the automated synthesizer (ABI 433A). DNA capture experiments by PNA was used to develop a method for PNA-mediated purification of genomic Chlamydia DNA from urine. This purification removed efficiently substances that impeded DNA amplification.Keywords
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