Electrophoretically detected germinal mutations induced in the mouse by ethylnitrosourea.
- 1 May 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (5) , 3138-3141
- https://doi.org/10.1073/pnas.78.5.3138
Abstract
Male DBA/2J and C57BL/6J mice were treated with ethylnitrosourea (EtNU) at a dose of 250 mg/kg of body wt. After a sterile period of 11 wk, the treated animals were mated with untreated females, treated DBA with C57 and treated C57 with DBA. A small control group of untreated males was established and a larger control group from earlier experiments was used for comparison. Tissue samples removed surgically from the parents and F1 offspring were examined by electrophoresis. Nine newly arisen mutants attributable to induction by EtNU were found in 22,512 loci tested for mutation. No mutations were encountered in 290,252 control locus tests. A preliminary study conducted concomitantly with the electrophoretic analyses for dominant lethal effects and physical malformation showed no increase over background due to EtNU treatment of spermatogonia; mutations induced by EtNU apparently are not expressed in this way. The nature of the mutants identified by electrophoresis facilitates follow-up analyses leading to increased understanding of the molecular basis and the physiological effects of induced mutations in mammalian organisms.This publication has 14 references indexed in Scilit:
- Mouse spermatogonia exposed to a high, multiply fractionated dose of a cancer chemotherapeutic drug: Mutation analysis by electrophoresisMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1981
- Rate of spontaneous mutation at human loci encoding protein structure.Proceedings of the National Academy of Sciences, 1980
- Search for mutations affecting protein structure in children of atomic bomb survivors: preliminary report.Proceedings of the National Academy of Sciences, 1980
- Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse.Proceedings of the National Academy of Sciences, 1979
- The relation between reaction kinetics and mutagenic action of mono-functional alkylating agents in higher eukaryotic systemsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- The specific locus test in the mouseMutation Research/Environmental Mutagenesis and Related Subjects, 1975
- Mutagenic effects of repeated small radiation doses to mouse spermatogonia I. Specific-locus mutation ratesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- The induction by X-irradiation of dominant lethal mutations in spermatogonia of miceMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1965
- Induction of Mutations in Mice by Chronic Gamma Irradiation; Interim ReportThe British Journal of Radiology, 1956
- X-RAY-INDUCED MUTATIONS IN MICECold Spring Harbor Symposia on Quantitative Biology, 1951