Four new electrophoretically slow‐moving glucose 6‐phosphate dehydrogenase variants associated with congenital nonspherocytic hemolytic anemia found in Japan: Gd(‐) Kurume, Gd(‐) Fukushima, Gd(‐) Yamaguchi and Gd(‐) Wakayama

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Abstract
Four new glucose 6‐phosphate dehydrogenase (G6PD) variants which had electrophoretically slow‐moving characteristics associated with congenital nonspherocytic hemolytic anemia were discovered in Japan. Gd(‐) Kurume was found in a 17‐year‐old male whose red cells contained only 0.8% of normal enzyme activity. Gd(‐) Kurume had a normal Km G6P, normal Km NADP, low Ki NADPH, normal utilization of 2‐deoxy‐G6P and deamino‐NADP, very low heat stability and a biphasic pH curve. The hemolytic anemia was mild. Gd(‐) Fukushima was characterized from a 33‐year‐old male and had 2.8% of normal enzyme activity, normal Km G6P, normal Km NADP, low Ki NADPH, normal utilization of 2‐dcoxy‐G6P, low utilization of deamino‐NADP, low heat stability and a normal pH curve. The hemolytic anemia was mild. Gd(‐) Yamaguchi was characterized from an 8‐year‐old boy and had 3.5% of normal enzyme.