A Novel Pathway for the Synthesis of Solanidine in the Isolated Chloroplast from Greening Potatoes

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Abstract
Synthesis of chlorophyll always preceded the synthesis of solanine in cold‐stored potatoes in the presence of dim light (30–40 lux). Isolated chloroplasts from green peelings were able to fix C02. The evidence obtained showed a direct reduction of CO2 to formate, which, was the primary product of fixation. Apart from this the chloroplasts were capable of incorporating NaH14C03, [14C]formate, [2‐14C]glycine, [2‐14C]pyruvate, [2‐14C]acetate, [2‐14C]mevalonate and [U‐14C]serine into the sol1tnidine moiety of the alkaloid. The intermediates in the pathway between CO2 and acetate have been identified as formate, glycine, serine and pyruvate. The localization of the enzymes involved in the reaction sequence viz. serine hydroxymethyl‐transferase, and pyruvic dehydrogenase complex, in the isolated chloroplast has been established.