Purification and characterization of cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii
- 30 January 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 166 (2) , 293-297
- https://doi.org/10.1016/0014-5793(84)80098-8
Abstract
A cell wall lytic enzyme of Chlamydomonas reinhardtii has been purified and identified as a single glycopolypeptide subunit of 62 kDa by SDS—polyacrylamide gel electrophoresis. It is released into culture medium by mating gametes as a large aggregate of subunits. The purified enzyme shows a pH optimum at about 7.5 and 35°C. Metal ion chelators and SH‐blocking agents inhibit the activity. The activity is also diminished by α2‐macroglobulin.Keywords
This publication has 20 references indexed in Scilit:
- A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gelsPublished by Elsevier ,2004
- Biased and non-biased transmission of chloroplast genes in somatic fusion products of Chlamydomonas reinhardtiiCurrent Genetics, 1983
- Transient increase in calcium efflux accompanies fertilization in Chlamydomonas.The Journal of cell biology, 1983
- Carbonic Anhydrase in Chlamydomonas reinhardtii I. LocalizationPlant and Cell Physiology, 1983
- Study of the release of cell wall degrading enzymes during adhesion of Chlamydomonas gametes*1Experimental Cell Research, 1982
- Two tunicamycin-sensitive components involved in agglutination and fusion ofChlamydomonas reinhardtii gametesArchiv für Mikrobiologie, 1982
- Biochemical studies of the excitable membrane of Paramecium tetraurelia VI. Endogenous protein substrates for in vitro and in vivo phosphorylation in cilia and ciliary membranes.The Journal of cell biology, 1981
- Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid ProteinsScience, 1981
- Evidence for role of flagella as sensory transducers in mating of Chlamydomonas reinhardiNature, 1977
- Self-assembly of a plant cell wall in vitroJournal of Molecular Biology, 1975