Density gradient separation of chicken thymus cells

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Abstract
Chicken thymus cells were separated into six fractions by a discontinuous albumin gradient centrifugation. When 10 or 16‐week old cell donors were used, thymus cells responding with antibody formation to Brucella abortus were clearly separated from those active in graft‐vs.‐host assays. The former were found in low and the latter in high density fractions. The results suggest that B and T cells in chicken thymus may be preferentially concentrated into separate fractions according to density.

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