Practical procedure for enzyme immunoassay of progesterone in bovine serum
- 1 February 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 93 (2) , 223-227
- https://doi.org/10.1530/acta.0.0930223
Abstract
: An enzyme immunoassay of progesterone was established by using β-galactosidase from E. coli as a label. The enzyme was conjugated with 11α-hydroxyprogesterone-hemisuccinate using water-soluble carbodiimide. Rabbit antiserum to 11α-hydroxyprogesterone-hemisuccinate-bovine serum albumin was previously obtained and anti-rabbit gamma globulin goat serum was used as second antibody. The enzyme activity was measured by utilizing hydrolysis of O-nitrophenyl-β-D-galactopyranoside. The least detectable concentration of progesterone was 12 pg per tube. The measurable range of progesterone in 0.1 ml of bovine serum was between 0.25 ng/ml and 10 ng/ml. This method satisfied the general criteria regarding specifity, precision and recovery rate. Correlation between the progesterone levels determined by enzyme immunoassay and radioimmunoassay was quite high (r = 0.99, P ≦ 0.01). The present enzyme immunoassay can be applied for practical and routine analysis of serum progesterone.This publication has 4 references indexed in Scilit:
- A specific enzymeimmunoassay for progesterone in human plasmaSteroids, 1977
- Enzyme-Labelled Immunoassay for Plasma CortisolJournal of Clinical Endocrinology & Metabolism, 1977
- Enzyme Immunoassay of Testosterone Using the Testosterone-Glucoamylase ComplexThe Journal of Biochemistry, 1976
- An immuno-enzymatic assay of cortisol using E. coli β-galactosidase as labelJournal of Immunological Methods, 1976