Inhibition of calcineurin by cyclosporin A‐cyclophilin requires calcineurin B
- 7 December 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 314 (1) , 37-40
- https://doi.org/10.1016/0014-5793(92)81456-v
Abstract
The interaction of the immunosuppressive complex cyclosporin A-cyclophilin (CsA-CyP) with the Ca2+/calmodulin-dependent protein phosphatase calcineurin is investigated using a recombinant form of the A subunit of calcineurin (rCNA). Only in the presence of purified calcineurin B (CNB) does rCNA show the response of native calcineurin, i.e. 50% inhibition of rCNA phosphatase activity at 6 nM human cyclophilin B and 0.6 μM human cyclophilin A using [32P]casein as substrate, yet stimulation of activity with p-nitrophenyl phosphate as substrate. This study demonstrates that the B subunit is necessary to confer sensitivity of calcineurin to CsA-CyP.Keywords
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