Plasmid R1 DNA replication dependent on protein synthesis in cell-free extracts of E. coli
- 1 January 1981
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 289 (5795) , 326-328
- https://doi.org/10.1038/289326a0
Abstract
Extracts of E. coli can carry out the complete replication of miniplasmids derived from the antibiotic-resistance plasmid R1. This R1 replication system differs from the previously described CoE1 and R6K systems in its strict dependence on DNA-directed protein synthesis. This is probably the 1st report of the functional coupling of the 3 fundamental reactions of genetic information transfer (transcription, translation and replication) in a cell-free system.Keywords
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