SOME ENZYMATIC ACTIVITIES OF A PARTICULATE FRACTION FROM SONIC LYSATES OF ESCHERICHIA COLI

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Abstract
An investigation of enzymatic activities of a cytochrome-containing particulate fraction from sonic lysates of E. coli is described. Of a fairly large number of substances investigated, only succinate, lactate, iormate, alpha-glycerophosphate, molecular hydrogen, and reduced diphosphopyridine nucleotide are oxidized by the particles. Nitrates, neotetrazolium, and methylene blue are reduced by the particles, but nitrites, p-nitrobenzoic acid, and furacin are not. The particles also showed sluggish catalase, formic hydrogenlyase, and ATPase activity but no phosphatase or lipase activity. Menadione apparently can serve as a hydrogen and electron carrier between flavoproteins and the cytochrome-containing particles. The effect of a number of knovvn enzyme inhibitors and antibacterial substances on succinate oxidation by the particles was also investigated.