Characterisation of the α1-protease inhibitor system in Thoroughbred horse plasma by horizontal two-dimensional (ISO-DALT) electrophoresis. 1. Protein staining

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Abstract
The isoelectric points and the MW of the major components of the 8 Thoroughbred protease inhibitor (Pi) types were determined by polyacrylamide gel isoelectric focusing and polyacrylamide gel pore gradient (ISO-DALT) electrophoresis, respectively. The major Pi proteins focus in the range pH 3.74-4.43 and have MW ranging from 55,000-72,000 daltons. Using the ISO-DALT method of electrophoresis, protein maps for the 8 Thoroughbred Pi types were presented for the first time. None of the homozygous Pi types are identical except for the types S1 and S2 which show partial identity. The results do not necessarily support Juneja et al.''s contention of 2 closely linked .alpha.1 Pi systems based on MW differences. The traditional nomenclature originally proposed by Braend be maintained to describe the 8 Pi alleles in Thoroughbred horse plasma. The ISO-DALT method provides a sensitive technique which is superior to existing techniques for the analysis of the horse Pi system.