Fatty Acid Composition of the Lipid Fractions from Bovine Serum Lipoproteins

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Abstract
Weights and fatty acid composition of the lipid fractions have been determined from bovine serum lipoproteins separated ultracentrifugally at a solvent density 1.063 g/ml and corresponding in electrophoretic mobility to a- and 0-lipoproteins. The vast proportion of serum lipids was contained in the high-density lipoproteins (D > 1.063) with the marked exception of the triglycerides which were evenly divided. Degree of saturation in the high-density lipoprotein total lipids was 27% and ranged from cholesteryl esters 8.4%, phospholipids 50%, non-esterified fatty acids 65%, to triglycerides 80%. The low-density lipoproteins (D<1.063) showed a 74% degree of saturation in their total lipids which varied from phospholipids 73%, triglycerides 84%, non-esterified fatty acids 86% to cholesteryl esters 89%. Fatty acid distribution indicated a high concentration of linoleate in the total lipids and cholesteryl esters of the high-density lipoproteins. This concentration was dispersed in their other lipid fractions. The low-density lipoproteins contained chielly palmitate and stearate in all lipid fractions. Triglycerides in both high- and low-density lipoproteins were notably similar in weight, saturation, and fatty acid distribution.