Studies on the amide and C-terminal residues in proteins. 2. The ammonia nitrogen and amide nitrogen of various native protein preparations

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Abstract
Attention is drawn to the need to ascertain the free NH3 content of native protein preparations as a factor in the determination of the amide N. A sample of ox insulin prepared by countercurrent distribution had a theoretical amide N of 6 groups/mole (5732 g); all other samples examined had low values, which suggested loss during manufacture from the pancreas.