Cannabidiol Prevents Cerebral Infarction Via a Serotonergic 5-Hydroxytryptamine 1A Receptor–Dependent Mechanism
- 1 May 2005
- journal article
- research article
- Published by Wolters Kluwer Health in Stroke
- Vol. 36 (5) , 1071-1076
- https://doi.org/10.1161/01.str.0000163083.59201.34
Abstract
Background and Purpose— Cannabidiol has been reported to be a neuroprotectant, but the neuroprotective mechanism of cannabidiol remains unclear. We studied the neuroprotective mechanism of cannabidiol in 4-hour middle cerebral artery (MCA) occlusion mice. Methods— Male MCA occluded mice were treated with cannabidiol, abnormal cannabidiol, anandamide, methanandamide, cannabidiol plus capsazepine, and cannabidiol plus WAY100135 before and 3 hours after MCA occlusion. The infarct size was determined after 24 hours (2,3,5-triphenyltetrazolium chloride staining). Cerebral blood flow (CBF) was measured at, before and 1, 2, 3, and 4 hours after MCA occlusion. Results— Cannabidiol significantly reduced the infarct volume induced by MCA occlusion in a bell-shaped curve. Similarly, abnormal cannabidiol but not anandamide or methanandamide reduced the infarct volume. Moreover, the neuroprotective effect of cannabidiol was inhibited by WAY100135, a serotonin 5-hydroxytriptamine1A (5-HT1A) receptor antagonist but not ...Keywords
This publication has 24 references indexed in Scilit:
- Neuroprotective effect of cannabidiol, a non‐psychoactive component fromCannabis sativa, on β‐amyloid‐induced toxicity in PC12 cellsJournal of Neurochemistry, 2004
- Selective Ligands and Cellular Effectors of a G Protein-Coupled Endothelial Cannabinoid ReceptorMolecular Pharmacology, 2003
- 5-Hydroxytryptamine 1A Receptor Activation Protects against N-Methyl-d-aspartate-Induced Apoptotic Cell Death in Striatal and Mesencephalic CulturesThe Journal of Pharmacology and Experimental Therapeutics, 2003
- Air PollutionStroke, 2002
- Molecular targets for cannabidiol and its synthetic analogues: effect on vanilloid VR1 receptors and on the cellular uptake and enzymatic hydrolysis of anandamideBritish Journal of Pharmacology, 2001
- ACTIVATION OF SEROTONERGIC 5-HT 1A RECEPTOR REDUCES Ca 2+ - AND GLUTAMATERGIC RECEPTOR-EVOKED ARACHIDONIC ACID AND NO/cGMP RELEASE IN ADULT HIPPOCAMPUSNeurochemistry International, 1996
- Neuroprotective Activity of HU‐211, a Novel Nonpsychotropic Synthetic CannabinoidAnnals of the New York Academy of Sciences, 1995
- Formation of Carbon Monoxide during Mouse Hepatic Microsomal Oxidative Metabolism of Cannabidiol; Identification and Determination.Biological & Pharmaceutical Bulletin, 1995
- A new method for continuous measurement of regional cerebral blood flow using laser Doppler flowmetry in a conscious ratNeuroscience Letters, 1994
- Vasodilator Effect of Tertatolol in Isolated Perfused Rat Kidneys: Involvement of Endothelial 5-HT1A ReceptorsCardiology, 1993