Advances in the Authenticity Assessment of Citrus Oils

Abstract
Chirality evaluation is proved to be an efficient tool for the authenticity control of neroli, petitgrain and bergamot oils by enantioselective multidimensional gas chromatography (enantio-MDGC). The simultaneous stereochemical analysis of the main compounds linalool, linalyl acetate, α-terpineol using heptakis-(2,3-di-o-acetyl-6-o-tert.-butyldimethyl-silyl)-β-cyclodextrin as the chiral main column is described. α-Pinene, β-pinene, limonene, terpinen-4-ol and nerolidol are simultaneously stereoanalyzed with heptakis-(2,3-di-o-methyl-6-o-tert.-butyldimethylsi-lyl)-β-cyclodextrin. Characteristic authenticity profiles of neroli, petitgrain, bergamot and other citrus oils are deduced by enantioselective cGC as well as isotope ratio mass spectrometry (IRMS), online coupled with capillary gas chromatography. Enantiomeric ratios, isotopic data as well as quantification of bergamot oil compounds are evaluated integrally. Scope and limitations of the techniques are discussed.

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