The Determination of Calcium, Glucose, Urea and Uric Acid Using the Kodak EKTACHEM Multilayer Film Technology: An Evaluation

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Abstract
Ca, glucose, urea and uric acid determinations on the Kodak Ektachem Four Chemistry Analyzer were tested for precision and an estimation of the accuracy was performed with routine laboratory methods using patients'' sera and control sera. Precision, examined over a period of 6 wk was very good. Even the very stringent claim of the College of American Pathologists (CAP) that analytical dispersion should be less than 1/16th of the normal range was fulfilled in almost all control samples. The results from patients'' sera measured using both the Ektachem and the routine laboratory methods showed very good correlation. Control sera tested for Ca, glucose and uric acid all resulted in values well within the assigned interval. Some negative bias was noted for the urea determination however. This could not be completely eliminated even though the samples were reconstituted with a bicarbonate solution according to the manufacturer''s instructions. The linear range of the 4 determinations was checked: Ca is linear up to 4.2 mmol/l, glucose up to 33 mmol/l, urea up to 60 mmol/l and uric acid up to 1370 .mu.ol/l. The long-term stability of the analyzer and slides were confirmed to be so high that 1 calibration/wk is sufficient.