Studies on the Isolation of Mitochondria from Potato Tuber Tissue

Publisher Website
Google Scholar
Abstract
A modified procedure is described for the isolation of mitochondria from potato tuber tissue. The respiratory behavior of these particulate fractions was recorded in the oxygen electrode test with succinate as the substrate. The rate of O2 uptake was controlled by ADP to a greater extent than in the preparations described in literature; the respiratory control ratios were roughly twice as high. The sub- strate oxidation was accelerated immediately after addition of ADP without any sign of a lag phase. It is concluded that the slow reaction to the addition of ADP found by Wiskich and Bonner was related to damage of the mitochondria in their preparations. With the modified procedure mitochondria could be isolated in a better condition.